Olive text indicates a specimen that has not been thoroughly examined (for example, for microscopic details) and marks other places in the text where data is missing or uncertain.
The following material is based on genetic sequences (including bacterial cloning) produced under the direction of Dr. K. W. Hughes (University of Tennessee, Knoxville) and other original research of R. E. Tulloss.
lamella edge tissue
A pair. Under large, old growth Pinus strobus.
U.S.A.: MAINE—York Co. - Old Orchard Beach, Ocean Park, Guild Pk. [43.5048 N/ 70.3919 W, 35 m], 28.viii.2009 Herbert Baker s.n. [mushroomobserver #24776] (RET 449-6, nrITS & nrLSU seq'd.)
The only material of this beautiful pink-capped species is immature.
It appears to be segregated from existing sequenced taxa by its nrLSU. With the most similar taxa (according to an nBLAST of GenBank data) being the western species A. breckonii and a collection identified as A. praecox by B. E. Wolfe.
The nrITS loci have not homogenized in this species; in order to have sample nrITS, bacterial cloning was required. Since this is not the case with the nrITS sequence obtained from the isotype of A. breckonii, the necessity of cloning distinguishes the present species from A. breckonii. In the cloning process, eight cultures produced four distinct sequences. The closest sequences in GenBank were from taxa presumed to be pantherinoid due to the species names applied to the near-match sequences.
Unfortunately, the nrITS sequences posted by Wolfe for his material identified as A. praecox have quality problems of unknown cause(s) including within the relatively conserved 5.8S region. Further research is clearly necessary.
Well-documented, mature material is needed in order to make further progress in describing the present taxon.
—R. E. Tulloss and K. W. Hughes
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can be found here.
1. Amanita sp-N60, York Co., Maine, U.S.A. (RET 449-6).
2. Amanita sp-N60, York Co., Maine, U.S.A. (RET 449-6).
3. Amanita sp-N60, York Co., Maine, U.S.A. (RET 449-6).
Herbert Baker - (1-3) York County, Maine, U.S.A. (RET 449-6). [Note: Original images are to be found on mushroomobserver.org, here.]
Spore data for collections provisionally identified as: Amanita sp-N60
Each spore data set is intended to comprise a set of measurements from a single specimen made by a single observer;
and explanations prepared for this site talk about specimen-observer pairs associated with each data set.
Combining more data into a single data set is non-optimal because it obscures observer differences
(which may be valuable for instructional purposes, for example) and may obscure instances in which
a single collection inadvertently contains a mixture of taxa.