Amanita sp-ITAL02

name

Amanita sp-ITAL02

author

Tulloss & S. D. Russell

name status

cryptonomen temporarium

GenBank nos.

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accession	locus	voucher	source

intro

The following text may make multiple use of each data field.

The field may contain magenta text presenting data from a type study and/or revision of other original material cited in the protolog of the present taxon. Macroscopic descriptions in magenta are a combination of data from the protolog and additional observations made on the exiccata during revision of the cited original material.

The same field may also contain black text, which is data from a revision of the present taxon (including non-type material and/or material not cited in the protolog). Paragraphs of black text will be labeled if further subdivision of this text is appropriate.

Olive text indicates a specimen that has not been thoroughly examined (for example, for microscopic details) and marks other places in the text where data is missing or uncertain.

The following material is based on collectors' notes and photographs; molecular studies of S. D. Russell and colleagues (Purdue Univ.), and additional original research by R. E. Tulloss.

stipe

?? × ?? mm, with up to 100 mm (more than 50% of stipe length) inserted in substrate, white, narrowing upward, sinuate or not, ??; context ??; exannulate; universal veil as saccate volva, membranous, persistent, with white exterior surface decorated with scattered rusty spots, with interior surface ??.

odor/taste

neither recorded.

macrochemical
tests

none recorded.

partial veil

absent.

lamella edge tissue

sterile.

ecology

Solitary or in small groups. At 537 - 713 m elev. With Castanea sativa or Quercus.

The primordia of this species form about 10 cm deep in the substrate below another 5 cm of living moss.

material examined

ITALY: TOSCANA—Pistoia - [44.0066° N / 10.93957° W, 713 m], 2.viii.2014 Luca Pasquali s.n. [mushroomobserver #172161 (RET 659-10, nrITS segments seq'd.), [43.9919° N / 10.9121° W, 527 m] 6.viii.2014 L. Pasquali s.n. [mushroomobserver #172698 ] (RET 660-1, nrITS segments seq'd.). .

discussion

This species is characterized genetically by (apparently consistently, but with low sample size) producing nrDNA in scattered segments characterized as follows:

One segment is 204 characters long and includes the complete 5.8S locus (161 characters) and terminal fragments of adjacent ITS1 and ITS2 loci.

Another is 202 characters long and contains a small piece of ITS2 and a 5' fragment of nrLSU including the "TCTGACCTCAAATCA" version of the nrLSU 5' motif. The sources of these fragments were an ITS4B read.

ITS1F reads from the same samples yielded 68 usable characters of which 22 were from nrSSU and the remainder from ITS1. This fragment is too small to be submitted to GenBank.

citations

—Tulloss & S. D. Russell

editors

RET

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name	Amanita sp-ITAL02
name status	cryptonomen temporarium
author	Tulloss & S. D. Russell
images	1. Amanita sp-ITAL02, Pistoia, Tuscano, Italia. (RET 659-10) 3. Amanita sp-ITAL02, Pistoia, Tuscano, Italia. (RET 660-1)
photo	Luca Pasquali - (1-2) Pistoia, Toscano, Italia. (RET 659-10). [Note: Untrimmed and unedited images may be found here] (3) Pistoia, Toscano, Italia. (RET 660-1). [Note: Untrimmed and unedited images may be found here]

Sporograph Form T.B.D.