1. Amanita americrocea, Minnesota, U.S.A. RET 266-2.
The description of this curious species, which suggests the European A. crocea, described from RET's observations. The original material examined was collected, photographed, and annotated by Dr. Anna Gerenday.
The cap is 70 - 122 mm wide, yellow-orange at first, then burnt orange to orange-tan and somewhat metallic, becoming shiny metallic on drying slightly in the field; it develops brown or olivaceous brown tints over the center. The cap is ovoid at first, then broadly campanulate, then broadly convex with a broad umbo. The cap flesh is white to cream except for yellow to orange under cap's skin. The cap margin is striate for less than a third to more than a half of the cap radius. Volval remnants are absent from the cap. The cap skin is deep orange in cross section even when the surface is dull and faded.
The gills are free, close to crowded, cream to off-white in mass and pale cream in side view. The short gills are truncate, unevenly distributed, of diverse lengths, and attached either to the stipe or the cap's margin.
The stem is 127 - 155 × 8 - 17.5 mm, with an off-white to cream ground color; it narrows upward, flares at the stem's top at maturity, and has a cone-shaped base. Its surface is decorated with pallid to pale orange fibers above the volval sac, and these fibers become upward pointing fibrillose scales that rather rapidly turn orange-brown when handled. The stem's flesh is white to off-white or, sometimes, orange near the stem's surface. The stem is partially hollow or stuffed with tightly packed white material and has no ring. There is a sack-like volva at the stem's base. The volva is membranous, smooth, white with orange-brown stains or spots on outside and has an inner surface that has a color which is a pallid variant of the color of the cap. The volva measures 25 - 59 mm high and up to 35 mm wide.
The odor and taste have not yet been recorded.
The spores of this species measure (7.4-) 8.3 - 12.5 (-20) × (6.8-) 7.2 - 11.5 (-14.5) μm and are globose to broadly ellipsoid (rarely ellipsoid) and inamyloid. No clamps are present at bases of basidia.
To date this provisional species is only known from the U.S.A. (Colorado, Minnesota, and Wyoming) where its reported woody plant associates are Aspen (Populus tremuloides, Birch (Betula), Oak (Quercus), and Pine (e.g., Pinus strobus).
elision of "America" and "crocea"; hence, American crocea
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The following is based upon
original research by R. E. Tulloss.
70 - 122 mm wide, yellow-orange at first, then burnt orange to orange-tan (near ISCC-NBS 71 M OY) to 7.5YR 8/6 and somewhat metallic, developing brown or olivaceous brown tint over disc, ovoid at first, then broadly campanulate with decurved margin, then broadly convex with broad umbo, dry, shiny over margin, becoming dull and hispidulose (having short fine and somewhat sparsely distributed upstanding hairs, under 10× lens) over disc within region of 35± mm diameter, becoming metallic shiny on drying slightly in situ; context white to cream except for yellow to orange under pileipellis, not changing when cut or bruised, 5 - 11 mm thick at stipe, thinning evenly to margin or very near it; margin striate [0.3R (-0.6R)], nonappendiculate; universal veil absent; pileipellis deep orange in cross section even when surface dull and faded.
free, close to crowded, cream to off-white in mass, pale cream in side view, unchanging when cut or bruised, drying pale orangish cream, 3 - 8.5 mm broad, with last few mm of edge nearest pileus margin concolorous with pileus surface; lamellulae truncate, unevenly distributed, of diverse lengths, attached to either stipe or pileus margin, plentiful in some sectors, not plentiful in others.
127 - 155 × 8 - 17.5 mm, with off-white to cream ground, narrowing upward, flaring at apex at maturity, with obconic base, having surface decorated with pallid to pale orange fibrils above volval sac, becoming upward pointing fibrillose scales, with these scales very rapidly becoming orange-brown from handling; context white to off-white, sometimes orange under stipipellis, partially hollow or stuffed with tightly packed white material, with central cylinder 3 - 11 mm wide; exannulate; universal veil as saccate volva, membranous, smooth, exterior white with orange-brown stains or spots, inner surface palely concolorous with pileus and orangish at least in upper portion as dried, 25 - 59 × ? - 35 mm, 1.5 - 2.5 mm thick at midpoint of limb, with point of attachment rather low? on stipe; limbus internus ?.
85 - 140 µm thick, yellow-orange, gelatinized only just at surface; filamentous, undifferentiated hyphae ? µm wide, ?, in subradially oriented fascicles criss-crossed by other fascicles; vascular hyphae 4.8 - 16.5 µm wide, branching, yellow to yellow-orange to deep orange, common, occasionally sinuous; no clamps observed.
wst-near = ? µm; wst-far = ? µm; cellular, with cells up to 24 × 17.5 µm, apparently including uninflated hyphal segments, with at least 2 to 4 cells between subhymenial base and base of nearest basidium/-ole in local region, with 3 to 6 cells between subhymenial base and base of most distant basidium/-ole in local region, with basidia arising from inflated cells ??.
40 - 53+ × (7.5-) 8.5 - 12.5 (-13.0) µm, 2- and 4-sterigmate, with sterigmata up to ? × ? µm; clamps not observed.
On pileus: absent. On stipe base, exterior surface: as layer 20 - 30 µm thick; filamentous, undifferentiated hyphae 2.3 - 6.1 µm wide, branching, interwoven with many proportionately large gaps through which interior visible, partially to largely gelatinized just in surface hyphae, otherwise barely or not at all gelatinized, without dominant orientation; vascular hyphae not observed. On stipe base, interior: filamentous, undifferentiated hyphae 2.3 - 10.2 µm wide, branching, interwoven (singly or in fascicles) in open lattice structure, occasionally with yellowish subrefractive walls, occasionally with narrowly clavate (e.g., 13 × 16.5 µm) to narrowly fusiform intercalary segments, with some tip segments very narrowly clavate (e.g., 61 × 12.7 µm); inflated cells terminal, thin-walled, narrowly clavate to clavate to subovoid (up to 100 × 47 µm or larger), scattered to rather common, apparently occasionally in small clusters; vascular hyphae not observed. On stipe base, inner surface: predominantly like interior, but slightly gelatinized, with scant remains of thin layer of hyphae present as isolated groups or small patches of fascicles, with such remains yellow-brown or brown-orange and often extensively gelatinized, with some hyphae in such remains having dominantly sublongitudinal oriented and others disoriented comprising interwoven fascicles.
lamella edge tissue
filamentous differentiated hyphae in fascicles 7 - 8 hyphae wide running parallel to lamella edge; inflated cells in scattered clusters 2-5 cell deep (in facial view of lamella), partially gelatinized, collapsed.
[97/5/4] (7.4-) 8.3 - 12.5 (-20) × (6.8-) 7.2 - 11.5
(-14.5) μm, (L = 8.8 - 9.8 (-11.4) μm;
L' = 9.9 μm; W = 7.9 - 9.1 (-10.0) μm;
W' = 8.9 μm; Q = (1.02-) 1.03 - 1.22 (-1.36);
Q = 1.07 - 1.15; Q' = 1.11), hyaline,
colorless, thin-walled, smooth, inamyloid, globose to
subglobose to broadly ellipsoid, infrequently
ellipsoid, occasionally lachrymiform, often
adaxially flattened; apiculus sublateral,
truncate conic; contents mono- to
multiguttulate with or without additional small
granules; white in deposit.
Solitary to subgregarious. Colorado: At up to 1990± m elev. Under conifers. Minnesota: In sandy soil near Betula with scattered Pinus strobus and Quercus sp. throughout area of alternating sand islands and wetlands with old, well-decayed trunks of Pinus and Quercus scattered through wooded parts. South Dakota: In grassy clearing of P. contorta forest with young trees. Wyoming: With Populus tremuloides.
COLORADO—Douglas Co. - Pike Nat. For., Indian Crk. Campground. [39°22'31" N/ 105°05'53"W], 17.viii.1991 Jerry Lavelle s.n. (DBG 18864).
MINNESOTA—Anoka Co. - Ham Lk., ix.1997 D. Johannes s.n. [A. Gerenday 1024] (MPPD; RET 271-9). Chisago Co. - E of Stacy, Carlos Avery Wildlife Refuge, ca. Sunrise R., 9.viii.1997 Anna Gerenday 1023 (MIN; RET 266-2). Unkn Co. - unkn. loc., 26.ix.1994 member Minn. Mycol. Soc. s.n. (RET 136-3).
NORTH CAROLINA—Transylvania Co. - Dupont St. For., 2nd tr. to waterfall, 15.viii.2009 J. Justice NC-AM-19 (RET 452-2).
WYOMING—Teton Co. - Teton Nat. Pk., Reid Mtn., 13.viii.1997 Joseph Ammirati s.n. [Tulloss 8-13-97-JA] (RET 265-6).
This species is distinguished from A. pseudocrocea by having slightly larger spores (see comparative sporographs below), having a somewhat thicker pileipellis, lacking a robust (pileipellis-like) inner surface layer of the universal veil at the stipe base, and having the longest marginal striations of any taxon in the "crocea group."
For purposes of comparison, spore data (red figure) for A. crocea are presented here: [521/27/18] (8.0-) 9.4 - 11.8 (-18.8) × (7.5-) 8.5 - 11.0 (-16.0) µm, (L = (9.8-) 10.0 - 11.1 (-11.3) µm; L’ = 10.6 µm; W = (8.9-) 9.1 - 10.5 µm; W’ = 9.7 µm; Q = (1.01-) 1.04 - 1.16 (-1.28); Q = 1.07 - 1.10 (-1.11); Q’ = 1.10).
For purposes of comparison, spore data (green figure) for A. pseudocrocea are presented here: [120/5/4] (7.8-) 8.6 - 10.8 (-21) × (6.4-) 7.2 - 9.5 (-17.8) µm, (L = 9.2 - 10.1 µm; L’ = 9.7 µm; W = 7.5 - 9.2 µm; W’ = 8.4 µm; Q = (1.05-) 1.06 - 1.30 (-1.59); Q = 1.10 - 1.20; Q’ = 1.15).
Amanita americrocea differs from all the European taxa in this group as well. Besides the marginal striations mentioned above, the present species has a thinner pileipellis than any of the European taxa. Of the European taxa, it appears most similar to A. romagnesiana Tulloss, but that species apparently lacks vascular hyphae in the pileipellis as well as having the other differences cited for European taxa in general.
For purposes of comparison, spore data (blue figure) for the neotype of A. romagnesiana are presented here: [40/1/1] (8.0-) 9.5 - 12.0 (-16.0) × (7.5-) 8.5 - 10.8 ( 14.2) µm, (L = 10.8 µm; W = 9.5 µm; Q = (1.07-) 1.09 - 1.22 (-1.31); Q = 1.14).
In mid-August, 2010, a collection that fits the provisional description of A. barrowsii A. H. Sm. nom. prov was photographed by Noah Siegel at the NAMA foray in Colorado [see mushroomobserver.org observation #50248]. This called RET's attention to the striking similarity between that provisional taxon and the present one. This similarity will be the subject of continuing research. If, indeed, it is found that the two provisional names apply to a single taxon, then RET's preference will be to use Smith's name—A. barrowsii.
DBG 18865 (not listed in the material examined) is likely to belong to the present species based on macroscopic characters, the minute hairs over the pileipellis (seen in dried material), the typical branching vascular hyphae in the pileipellis, and the collection area ("Front Range," Colorado).
Amanita americrocea has appeared in my correspondence, keys, and foray lists as Amanita sp. MN1 and A. sp. WY2.
—R. E. Tulloss
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"Western American Saffron Ringless Amanita"
1. Amanita americrocea, Minnesota, U.S.A. RET 266-2.
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